By the nature of his work, a dentist has a higher risk of tuberculosis infection than the average Dutch population. Thus, the question arises whether dentists do have sufficient knowledge on tuberculosis. In order to determine their knowledge, an inventory was conducted among a sample of dentists in the Netherlands. Analysis of the dentists’ response to 19 correct or incorrect statements revealed that their knowledge level on tuberculosis was rather low, did not vary with regard to gender, and was independent of the region where they were practising and of their explicit medical interest. The dentists indicated a desire for education on tuberculosis.